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CPM Seminar
Jürgen Fritz
Novartis Scientific Services, Physics, Basel, Switzerland The AFM technique has many applications in life sciences. Because of its high sensitivity and spatial resolution single biomolecules can be imaged and addressed in real space as will be shown for cell adhesion proteoglycans. AFM allows to measure rupture forces and elasticities of single molecules, what is especially interesting for systems which were designed by nature to interact under external forces. By stretching single ligand/receptor complexes the interplay between elasticity, rupture force and lifetime of a molecular binding can be studied, for example in a selectin system. Furthermore, the AFM cantilevers per se can be used as a biosensing tool to monitor molecular interactions by changes of surface stress resulting in a deflection of the cantilever.
Tuesday, March 10th, 13:15 |